Article

Temporal changes in skin and gill microbiomes of Atlantic salmon in a recirculating aquaculture system – Why do they matter?

Details

Citation

Lorgen-Ritchie M, Clarkson M, Chalmers L, Taylor JF, Migaud H & Martin SAM (2022) Temporal changes in skin and gill microbiomes of Atlantic salmon in a recirculating aquaculture system – Why do they matter?. Aquaculture, 558, Art. No.: 738352. https://doi.org/10.1016/j.aquaculture.2022.738352

Abstract
Mucosal surfaces are key components of teleost health, providing defence against opportunistic pathogens and other insults. Maintaining the integrity of mucosal surfaces and their associated microbial communities, especially the gill and skin that have large surface areas exposed to the environment is essential. Production of Atlantic salmon in land-based recirculating aquaculture systems (RAS) has increased significantly in recent years as it allows greater control over stability of the environment in which fish are reared, reduces water demand and minimises environmental impacts. However, little is known about the impact of the RAS environment upon the temporal dynamics of skin and gill mucosal microbiomes. In this study we examined microbial communities in gill mucus, skin mucus and rearing water throughout freshwater (FW) RAS production, and at 1-week and 4-weeks following transfer to seawater (SW) in open cage production using 16S rRNA sequencing. Microbial diversity and richness in skin and gill mucus of fish reared in a RAS system were temporally dynamic. Dynamics in richness and diversity were similar in the two mucosal tissues, and to some extent also mirrored that of the surrounding water. Dysbiosis indicated by an abrupt decline in diversity during FW production coincided with an increase in the relative abundance of two taxa belonging to the RAS-biofilter-associated nitrogen-cycling genus Hydrogenophaga in RAS tank water and this was also observed in gill and skin mucus. Extensive overlap in core taxa was observed between gill and skin mucus, but host-specific cores were non-existent during the dysbiotic event with all cores present in the rearing water. Diversity remained stable during the transition from FW to SW, but distinct community composition and core taxa were observed in the two environments. Although RAS are closely controlled, significant temporal variation could be observed in temperature as well as levels of CO2 and nitrogen compounds, reflecting the increasing biological load within the system over time. The results presented here suggest that, in terms of microbiomes, dysbiosis may occur in both the RAS environment and fish mucosal surfaces over time, but microbial communities have the capability to recover.

Keywords
Atlantic salmon; Gill; Microbiome; Recirculating aquaculture system (RAS); Skin; Temporal dynamics

Journal
Aquaculture: Volume 558

StatusPublished
FundersScottish Aquaculture Innovation Centre, Biotechnology and Biological Sciences Research Council and BBSRC Biotechnology and Biological Sciences Research Council
Publication date15/09/2022
Publication date online14/05/2022
Date accepted by journal11/05/2022
URLhttp://hdl.handle.net/1893/34450
PublisherElsevier BV
ISSN0044-8486

People (1)

Professor Herve Migaud

Professor Herve Migaud

Honorary Professor, Institute of Aquaculture

Projects (1)