My main research aim is to develop new analytical methodologies, which can be used to explore lipid metabolism in greater detail within commercially important aquaculture species, such as Salmon. Such methodologies are required to establish the parity between traditional feed formulations and those of novel feed ingredients, such as insects and GM oils, which may allow for more sustainable aquaculture. My work has included establishing a lipidomics platform for understanding the isomeric alterations that occur within Salmon, which have been fed feed containing novel terrestrial sources of omega-3.
My research interests generally focus around lipids, however I also develop methods for more targeted analysis, such as phytochemicals and compounds commonly utilised within the aquaculture industry. Common techniques include LC-MS/MS (Triple quad and QTof), HPLC and GC-MS. I also develop commercial methods for both food and human blood spot analysis, which has included Vitamin D and 25-OH Vitamin D from seafood and consumer blood spot testing kits.
Article
Wischhusen P, Heraud C, Broughton R, Surget A, Lanuque A, Betancor M, Terrier F & Fontagné-Dicharry S (2024) Vitamin B6 and selenium supplementation induce contrasting effects in the transsulfuration pathway of juvenile rainbow trout (Oncorhynchus mykiss) with interactive effects in stressed fish. Aquaculture, 593, Art. No.: 741354. https://doi.org/10.1016/j.aquaculture.2024.741354
Article
Maar K, Papadimitraki M, Hernández Ruiz L, Broughton R & Jónasdóttir S (2023) Lipids in meso- and bathypelagic fishes from the North Atlantic Ocean: dietary inputs suggested from fatty acid trophic markers. Marine Ecology Progress Series, 717, pp. 127-141. https://doi.org/10.3354/meps14366
Article
Implication of adipocytes from subcutaneous adipose tissue and fatty
Carlos Campos-Sánchez J, Gonzalez-Silvera D, Gong X, Broughton R, Guardiola FA, Betancor MB & Ángeles M (2022) Implication of adipocytes from subcutaneous adipose tissue and fatty. Fish and Shellfish Immunology, 131, pp. 160-171. https://doi.org/10.1016/j.fsi.2022.09.066
Article
Broughton R, Tocher DR, Napier JA & Betancor MB (2022) Profiling Phospholipids within Atlantic Salmon Salmo salar with Regards to a Novel Terrestrial Omega-3 Oil Source. Metabolites, 12 (9), Art. No.: 851. https://doi.org/10.3390/metabo12090851
Article
Morro B, Broughton R, Balseiro P, Handeland SO, Mackenzie S, Doherty MK, Whitfield PD, Shimizu M, Gorissen M, Sveier H & Albalat A (2021) Endoplasmic reticulum stress as a key mechanism in stunted growth of seawater rainbow trout (Oncorhynchus mykiss). BMC Genomics, 22 (1), Art. No.: 824. https://doi.org/10.1186/s12864-021-08153-5
Book Chapter
Analysis of Free and Esterified Sterol Content and Composition in Seeds Using GC and ESI-MS/MS
Broughton R & Beaudoin F (2021) Analysis of Free and Esterified Sterol Content and Composition in Seeds Using GC and ESI-MS/MS. In: Bartels D & Dörmann P (eds.) Plant Lipids. Methods in Molecular Biology, 2295. New York: Springer US, pp. 179-201. https://doi.org/10.1007/978-1-0716-1362-7_11
Article
Broughton R, Tocher DR & Betancor MB (2020) Development of a C18 Supercritical Fluid Chromatography-Tandem Mass Spectrometry Methodology for the Analysis of Very-Long-Chain Polyunsaturated Fatty Acid Lipid Matrices and Its Application to Fish Oil Substitutes Derived from Genetically Modified Oilseeds in the Aquaculture Sector. ACS Omega, 5 (35), pp. 22289-22298. https://doi.org/10.1021/acsomega.0c02631
Article
Hill PW, Broughton R, Bougoure J, Havelange W, Newsham KK, Grant H, Murphy DV, Clode P, Ramayah S, Marsden KA, Quilliam RS, Roberts P, Brown C, Read DJ & Deluca TH (2019) Angiosperm symbioses with non-mycorrhizal fungal partners enhance N acquisition from ancient organic matter in a warming maritime Antarctic. Ecology Letters, 22 (12), pp. 2111-2119. https://doi.org/10.1111/ele.13399
