Field trial of a genetically improved baculovirus insecticide

Citation

Cory JS, Hirst ML, Williams T, Hails RS, Goulson D, Green BM, Carty TM, Possee RD, Cayley PJ & Bishop DHL (1994) Field trial of a genetically improved baculovirus insecticide. Nature, 370 (6485), pp. 138-140. https://doi.org/10.1038/370138a0

Abstract
IMPROVEMENT of biological pesticides through genetic modification has enormous potential and the insect baculoviruses are particularly amenable to this approach1,2. A key aim of genetic engineering is to increase their speed of kill, primarily by the incorporation of genes which encode arthropod or bacterially derived insect-selective toxins3–11, insect hormones12,13 or enzymes14,15. We report here the first, to our knowledge, field trial of a genetically improved nuclear polyhedrosis virus of the alfalfa looper, Autogmpha californica (AcNPV) that expresses an insectselective toxin gene (AaHIT) derived from the venom of the scorpion Androclonus australisl6–18. Previous laboratory assays with the cabbage looper, Trichoplusia ni, demonstrated a 25% reduction in time to death compared to the wild-type virus, but unaltered pathogenicity6 and host range19. In the field, the modified baculovirus killed faster, resulting in reduced crop damage and it appeared to reduce the secondary cycle of infection compared to the wild-type virus.

Journal
Nature: Volume 370, Issue 6485