Production and efficacy of an Aeromonas hydrophila recombinant S-layer protein vaccine for fish

Citation

Poobalane S, Thompson K, Ardo L, Verjan N, Han H, Jeney G, Hirono I, Aoki T & Adams A (2010) Production and efficacy of an Aeromonas hydrophila recombinant S-layer protein vaccine for fish. Vaccine, 28 (20), pp. 3540-3547. https://doi.org/10.1016/j.vaccine.2010.03.011

Abstract
A recombinant protein for the S-layer protein of Aeromonas hydrophila was produced and its ability to protect common carp Cyprinus carpio L. against six virulent isolates of A. hydrophila was assessed. A group of 120 carp (30-40 g) were vaccinated intra-peritoneally with 0.1 ml of adjuvanted vaccine (30 μg protein per fish). Another group of 120 carp were injected with 0.1 ml of PBS-adjuvant mixture to serve as controls. Twenty fish from each group were challenged with each one of six virulent isolates of A. hydrophila 35 days post-vaccination. The fish were maintained in 12 separate tanks before terminating the experiment at 16 days post-challenge. The relative percentage survival (RPS) for the six isolates of A. hydrophila ranged from 56 to 87%. The difference in survival rate of fish challenged with four of the isolates was statistically significant in vaccinated fish compared to control fish, when analysed using a Chi-square test. The results of the study suggest that the recombinant S-layer protein of A. hydrophila could be useful as a vaccine antigen to protect fish against different isolates of this pathogenic bacterium.

Keywords
Aeromonas hydrophila; Recombinant S-layer protein vaccine; Efficacy testing; Common carp; Aeromonas hydrophila;Fishes Diseases Vaccines

Journal
Vaccine: Volume 28, Issue 20