Genomic resources and microarrays for the common carp Cyprinus carpio L.

Citation

Williams DR, Li W, Hughes MA, Gonzalez SF, Vernon C, Vidal MC, Jeney Z, Jeney G, Dixon P, McAndrew B, Bartfai R, Orban L, Trudeau VL, Rodgers J, Matthews LH, Fraser EJ, Gracey AY & Cossins AR (2008) Genomic resources and microarrays for the common carp Cyprinus carpio L.. Journal of Fish Biology, 72 (9), pp. 2095-2117. https://doi.org/10.1111/j.1095-8649.2008.01875.x

Abstract
The common carp is an important fish species satisfying ornamental, food and recreational fisheries' needs worldwide, but in common with other cyprinid fishes, it is particularly renowned for its environmental tolerance. Investigating the mechanistic basis of growth, disease and environmental tolerance is greatly enhanced by access to a comprehensive list of gene sequences and post-genomic technologies. The current status of genomic resources is described for this species including 40 k cDNA clone collections, their associated expressed sequence tags (ESTs) and a developing series of 13 k-26 k cDNA microarrays fabricated from amplicons. The arrays have been directed at questions of response to environmental stress (cold and hypoxia), viral and bacterial disease and ectoparasite infection. Consequently, clones from a wide range of tissues were prepared. The authors discuss how these resources were generated and their application. Evidence is presented supporting that the carp microarray may also be useful as a heterologous set of probes in studies of other fish species. (C) 2008 The Authors Journal compilation (C) 2008 The Fisheries Society of the British Isles.

Keywords
40; access; BACTERIAL; British; C; CARP; cDNA; CDNA MICROARRAY; CLONES; common carp; Cyprinus carpio; CYPRINUS-CARPIO; Disease; evidence; fish; fisheries; fishery; FISHES; Food; gene; Growth; INFECTION; microarray; NEED; needs; other; Probes; RANGE; recreational fisheries; resources; SEQUENCE; SEQUENCES; SERIES; SOCIETIES; Society; Stress; Technology; TISSUE; Tolerance

Journal
Journal of Fish Biology: Volume 72, Issue 9