Proteomic analysis of rainbow trout (Oncorhynchus mykiss, Walbaum) serum after administration of probiotics in diets

Citation

Brunt J, Hansen R, Jamieson D & Austin B (2008) Proteomic analysis of rainbow trout (Oncorhynchus mykiss, Walbaum) serum after administration of probiotics in diets. Veterinary Immunology and Immunopathology, 121 (3-4), pp. 199-205. https://doi.org/10.1016/j.vetimm.2007.09.010

Abstract
The response of rainbowtrout (Oncorhynchusmykiss, Walbaum) towards probiotics present in the feed was investigated by examining the proteome of serum as a measure of the acute phase response (APR). Proteomicanalysis by two-dimensional electrophoresis (2D) concurrently with mass spectrometry was used to detect APR related proteins in rainbowtroutserum following feeding with probioticsAeromonas sobria GC2 and Bacillus sp. JB-1. Three candidate proteins increased following use of GC2, and were putatively identified as NADH dehydrogenase, dystrophin and mKIAA0350. Conversely, one of the proteins, which were induced following use of JB-1 was identified as transferrin.

Keywords
Probiotic; Fish disease; Acute phase response; Innate immunity; Proteomics

Journal
Veterinary Immunology and Immunopathology: Volume 121, Issue 3-4