Isolation of polymorphic microsatellites in the stemless thistle (Cirsium acaule) and their utility in other Cirsium species

Citation

Jump A, Dawson DA, James CM, Woodward FI & Burke T (2002) Isolation of polymorphic microsatellites in the stemless thistle (Cirsium acaule) and their utility in other Cirsium species. Molecular Ecology Notes, 2 (4), pp. 589-592. https://doi.org/10.1046/j.1471-8286.2002.00329.x

Abstract
The genus Cirsium includes species with both widespread and restricted geographical distributions, several of which are serious weeds. Nine polymorphic microsatellite loci were isolated from the stemless thistle Cirsium acaule. Eight were polymorphic in C. acaule, six in C. arvense and seven in C. heterophyllum. One locus monomorphic in C. acaule showed polymorphism in C. heterophyllum. The mean number of alleles per locus was 4.1 in C. acaule, 6.2 in C. arvense and 2.9 in C. heterophyllum. These nine loci were also amplified in C. eriophorum and C. vulgare, suggesting that these markers may be of use throughout the genus.

Keywords
Asteraceae; Cirsium; cross-species amplification; microsatellite; thistle; weed; Life Sciences; Plant breeding

Journal
Molecular Ecology Notes: Volume 2, Issue 4