An improved selective isolation medium for the recovery of Listeria monocytogenes from smoked fish

Citation

Neamatallah AAN, Dewar SJ & Austin B (2003) An improved selective isolation medium for the recovery of Listeria monocytogenes from smoked fish. Letters in Applied Microbiology, 36 (4), pp. 230-233. https://doi.org/10.1046/j.1472-765X.2003.01298.x

Abstract
Aims: The aim of this study was to improve the selective isolation of Listeria monocytogenes from smoked haddock fillets. Methods and Results: Listeria selective agar (LSA) - Oxford formulation was supplemented with 25 lg ml)1 of colistin sulphate and 30 lg ml)1 of nalidixic acid. Inocula from four smoked haddock fillets produced colonies (approx. 2-13 bacteria g)1), identified as L. monocytogenes, on LSA supplemented with antimicrobial compounds (MLSA). Moreover, there was only negligible evidence of bacteria which were not L. monocytogenes on MLSA. In contrast, LSA supported dense bacterial growth, which was not equated with L. monocytogenes. Significance and Impact of the Study: The modified medium permitted the recovery of L. monocytogenes from smoked haddock fillets and reduced the growth of contaminating bacteria.

Keywords
Listeria monocytogenes; Selective isolation; Smoked haddock

Journal
Letters in Applied Microbiology: Volume 36, Issue 4