Overexpression, purification, characterization, and pathogenicity of Vibrio harveyi hemolysin VHH

Citation

Zhong Y, Zhang X, Chen J, Chi Z, Sun B, Li Y & Austin B (2006) Overexpression, purification, characterization, and pathogenicity of Vibrio harveyi hemolysin VHH. Infection and Immunity, 74 (10), pp. 6001-6005. https://doi.org/10.1128/IAI.00512-06

Abstract
Vibrio harveyi VHH hemolysin is a putative pathogenicity factor in fish. In this study, the hemolysin gene vhhA was overexpressed in Escherichia coli, and the purified VHH was characterized with regard to pH and temperature profiles, phospholipase activity, cytotoxicity, pathogenicity to flounder, and the signal peptide.

Journal
Infection and Immunity: Volume 74, Issue 10