Polyunsaturated fatty acid metabolism in fish cells: differential metabolism of (n-3) and (n-6) series acids by cultured cells orginating from a freshwater teleost fish and from a marine teleost fish

Citation

Tocher DR, Carr J & Sargent JR (1989) Polyunsaturated fatty acid metabolism in fish cells: differential metabolism of (n-3) and (n-6) series acids by cultured cells orginating from a freshwater teleost fish and from a marine teleost fish. Comparative Biochemistry and Physiology - B: Comparative Biochemistry, 94 (2), pp. 367-374. https://doi.org/10.1016/0305-0491%2889%2990357-X

Abstract
1. 1. The incorporation and metabolism of (n-3) and (n-6) polyunsaturated fatty acids (PUFA) supplemented to growing cultures were studied in rainbow trout (RTG-2) and turbot (TF) cell lines. 2. 2. A fatty acid concentration of 20 μM considerably altered the fatty acid composition of the cells without affecting lipid class composition or the apperance of cytoplasmic lipid droplets. 3. 3. Both cell lines exhibited considerable Δ6 desaturase activities. 4. 4. Whereas Δ5 desaturase activity was expressed in TF cells, but there was an apparent deficiency in the C18 to C20 elongase multi-enzyme complex. 5. 5. The Δ6 desaturase activity in both cell lines showed little preference between 18:2(n-6) and 18:3(n-3) but the Δ5 desaturase activity of RTG-2 cells and the Δ4 desaturase activity of TF cells showed a preference for (n-3)PUFA. 6. 6. Two fish oil concentrates were assessed for their ability to generate fatty acid compositions in the cell lines more closely resembling those of intact fish tissues.

Journal
Comparative Biochemistry and Physiology - B: Comparative Biochemistry: Volume 94, Issue 2