Effects of growth factors on the metabolism of linolenate (18:3n-3) and eicosapentaenoate (20:5n-3) in rainbow trout (Oncorhynchus mykiss) astroglial cells in primary culture

Citation

Tocher DR (1993) Effects of growth factors on the metabolism of linolenate (18:3n-3) and eicosapentaenoate (20:5n-3) in rainbow trout (Oncorhynchus mykiss) astroglial cells in primary culture. Comparative Biochemistry and Physiology - B: Comparative Biochemistry, 105 (3-4), pp. 743-748. https://doi.org/10.1016/0305-0491%2893%2990115-L

Abstract
1. Non-confluent primary cultures of rainbow trout (Oncorhynchus mykiss) brain astroglial cells were treated with growth factors and the effects on n-3 polyunsaturated fatty acid (PUFA) metabolism were investigated. 2. The net incorporation of radioactivity into trout astroglial cell total lipid from 14C-20:5n-3 was significantly greater than the incorporation of radioactivity from 14C-18:3n-3 and was independent of the effect of growth factors. 3. Epidermal growth factor significantly increased the net incorporation of both 14C-18:3n-3 and 14C-20:5n-3 into total lipid. 4. The activities of the component enzymes of the desaturase/elongase pathway were greater in the non-confluent cultures compared with the activities in confluent cultures used in a previous study, although Δ4-desaturase, the specific enzyme responsible for the production of 22:6n-3 from 22:5n-3, was still the lowest of the desaturase activities. 5. Growth factors did not affect the overall extent of metabolism of 14C-18:3n-3 via the desaturase/elongase pathway, but the amount of 14C-20:5n-3 recovered unmetabolized was significantly decreased in cultures treated with endothelial cell growth supplement. 6. Treatment with growth factors had significant effects on the percentages of radioactivity recovered in various intermediates and products of the desaturase/elongase pathways perhaps reflecting differential effects on the component enzymes of the pathways.

Journal
Comparative Biochemistry and Physiology - B: Comparative Biochemistry: Volume 105, Issue 3-4