No relationship between morphology changes and metabolism of α-linolenate and eicosapentaenoate in rainbow trout (Oncorhynchus mykiss) astroglial cells in primary culture

Citation

Tocher DR & Sargent JR (1993) No relationship between morphology changes and metabolism of α-linolenate and eicosapentaenoate in rainbow trout (Oncorhynchus mykiss) astroglial cells in primary culture. Comparative Biochemistry and Physiology - Part C: Pharmacology, Toxicology and Endocrinology, 106 (1), pp. 211-219. https://doi.org/10.1016/0742-8413%2893%2990274-O

Abstract
1. Primary cultures of rainbow trout brain astroglial cells, prelabelled with [1-14C] polyunsaturated fatty acids (PUFA), were treated with various agents and the effects on cell morphology and n-3 PUFA metabolism were investigated. 2. The effects of dibutyryl cAMP on trout astroglial cell cultures were similar to those of dibutyryl cAMP on primary cultures of rat brain astroglia, inducing process-bearing morphology. 3. Hydrocortisone had the opposite effect to dibutyryl cAMP on the morphology of the trout astroglial cells, reducing the degree of process-bearing morphology. 4. Dibutyryl cAMP increased the percentage of [14C]eicosapentaenoic acid (EPA, 20:5n-3) elongated to 22:5, whereas hydrocortisone increased the percentage of [14C]linolenic acid (LNA, 18:3n-3) desaturated and elongated to 20:5. 5. Peroxisome effectors also affected trout astroglial cell morphology with the peroxisomal proliferator, clofibrate, reducing the degree of process formation, whereas the peroxisomal inhibitor, 3-aminotriazole, increased the degree of process formation. 6. However, the peroxisomal effectors had similar effects on n-3 PUFA metabolism, reducing the percentages of [14C]LNA converted to 20:5 and 22:5, whereas both increased the percentages of [14C]EPA converted to 22:5 and 22:6. 7. We concluded that changes in the morphology of trout astroglial cells in vitro are not directly related to changes in n-3 PUFA metabolism.

Journal
Comparative Biochemistry and Physiology - Part C: Pharmacology, Toxicology and Endocrinology: Volume 106, Issue 1