Primary culture of astrocytic glial cells from rainbow trout, Salmo gairdneri L., brain

Citation

Tocher DR & Wilson R (1990) Primary culture of astrocytic glial cells from rainbow trout, Salmo gairdneri L., brain. Journal of Neuroscience Methods, 33 (2-3), pp. 93-100. https://doi.org/10.1016/0165-0270%2890%2990013-6

Abstract
A method is described for the preparation and maintenance of rainbow trout brain astrocytes in primary culture. A dissociated cell suspension was prepared from brains from young fish by mechanical sieving through nylon guazes, and the cells cultured in polylysine-treated plastic tissue culture flasks at 22°C. The resultant cultures were characterised by specific immunofluorescent staining using glial fibrillary acidic protein (GFAP) for astrocytes and Thy 1 for fibroblastic cells. The cultures were > 95% astrocytes with fibroblasts the only other cell type present. These highly enriched astrocyte cultures provide a unique system for various neurochemical, neurophysiological and biochemical studies of fish neural cells. Polyunsaturated fatty acid metabolism will be an area of particular interest.

Keywords
Rainbow trout; Astrocytes; Primary culture; GFAP; Immunofluorescence

Journal
Journal of Neuroscience Methods: Volume 33, Issue 2-3