The influence of carbohydrate-protein co-ingestion following endurance exercise on myofibrillar and mitochondrial protein synthesis

Citation

Breen L, Philp A, Witard O, Jackman SR, Selby A, Smith K, Baar K & Tipton K (2011) The influence of carbohydrate-protein co-ingestion following endurance exercise on myofibrillar and mitochondrial protein synthesis. Journal of Physiology, 589 (16), pp. 4011-4025. https://doi.org/10.1113/jphysiol.2011.211888

Abstract
The aim of the present study was to determine mitochondrial and myofibrillar muscle protein synthesis (MPS) when carbohydrate (CHO) or carbohydrate plus protein (C+P) beverages were ingested following prolonged cycling exercise. The intracellular mechanisms thought to regulate MPS were also investigated. In a single-blind, cross-over study, 10 trained cyclists (age 29±6 years, ˙VO2max 66.5±5.1 ml kg-1 min-1) completed two trials in a randomized order. Subjects cycled for 90 min at 77±1% ˙V O2max before ingesting aCHO(25 g of carbohydrate) or C+P (25 g carbohydrate+10 g whey protein) beverage immediately and 30 min post-exercise. A primed constant infusion of L-[ring-13C6]phenylalanine began 1.5 h prior to exercise and continued until 4 h post-exercise. Muscle biopsy samples were obtained to determine myofibrillar and mitochondrial MPS and the phosphorylation of intracellular signalling proteins. Arterialized blood samples were obtained throughout the protocol. Plasma amino acid and urea concentrations increased following ingestion ofC+P only. Seruminsulin concentration increased more for C+P than CHO. Myofibrillar MPS was ∼35% greater for C+P compared with CHO (0.087±0.007 and 0.057±0.006% h-1, respectively; P =0.025).MitochondrialMPS rates were similar for C+P and CHO (0.082±0.011 and 0.086±0.018% h-1, respectively). mTORSer2448 phosphorylation was greater for C+P compared with CHO at 4 h post-exercise (P less than 0.05). p70S6KThr389 phosphorylation increased at 4 h post-exercise for C+P (P less than 0.05), whilst eEF2Thr56 phosphorylation increased by ∼40% at 4 h post-exercise for CHO only (P less than 0.01). The present study demonstrates that the ingestion of protein in addition to carbohydrate stimulates an increase in myofibrillar, but not mitochondrial, MPS following prolonged cycling. These data indicate that the increase in myofibrillar MPS for C+P could, potentially, be mediated through p70S6K, downstream of mTOR, which in turn may suppress the rise in eEF2 on translation elongation.

Journal
Journal of Physiology: Volume 589, Issue 16