Article
Details
Citation
Brown RC, Tsalavouta M, Terzoglou V, Magoulas A & McAndrew B (2005) Additional microsatellites for Sparus aurata and cross-species amplification within the Sparidae family. Molecular Ecology Notes, 5 (3), pp. 605-607. https://doi.org/10.1111/j.1471-8286.2005.01007.x
Abstract
Six new microsatellite loci were isolated and characterized in 32 individuals from a farm population of gilthead seabream (Sparus aurata). Expected heterozygosity at all loci was high, ranging from 0.835 to 0.958 with between 10 and 27 alleles per locus. A multiplex polymerase chain reaction protocol was developed using four of the loci. Cross-species amplification of the loci was tested in six species of the Sparidae family and four loci were successfully amplified in two or more related species.
Keywords
cross-species amplification; gilthead seabream; microsatellites; Sparidae; Sparus aurata
Journal
Molecular Ecology Notes: Volume 5, Issue 3
Status | Published |
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Publication date | 30/09/2005 |
URL | http://hdl.handle.net/1893/9633 |
Publisher | Wiley-Blackwell |
ISSN | 1471-8278 |