Preexercise aminoacidemia and muscle protein synthesis after resistance exercise

Citation

Burke LM, Hawley JA, Ross ML, Moore DR, Phillips SM, Slater GR, Stellingwerff T, Tipton K, Garnham AP & Coffey VG (2012) Preexercise aminoacidemia and muscle protein synthesis after resistance exercise. Medicine and Science in Sports and Exercise, 44 (10), pp. 1968-1977. https://doi.org/10.1249/MSS.0b013e31825d28fa

Abstract
Purpose: We have previously shown that the aminoacidemia caused by the consumption of a rapidly digested protein after resistance exercise enhances muscle protein synthesis (MPS) more than the amino acid (AA) profile associated with a slowly digested protein. Here, we investigated whether differential feeding patterns of a whey protein mixture commencing before exercise affect postexercise intracellular signaling and MPS. Methods: Twelve resistance-trained males performed leg resistance exercise 45 min after commencing each of three volume-matched nutrition protocols: placebo (PLAC, artificially sweetened water), BOLUS (25 g of whey protein + 5 g of leucine dissolved in artificially sweetened water; 1× 500 mL), or PULSE (15× 33-mL aliquots of BOLUS drink every 15 min). Results: The preexercise rise in plasma AA concentration with PULSE was attenuated compared with BOLUS (P less than 0.05); this effect was reversed after exercise, with two-fold greater leucine concentrations in PULSE compared with BOLUS (P less than 0.05). One-hour postexercise, phosphorylation of p70 S6Kthr389 and rpS6ser235/6 was increased above baseline with BOLUS and PULSE, but not PLAC (P less than 0.05); furthermore, PULSE greater than BOLUS (P less than 0.05). MPS throughout 5 h of recovery was higher with protein ingestion compared with PLAC (0.037 ± 0.007), with no differences between BOLUS or PULSE (0.085 ± 0.013 vs. 0.095 ± 0.010%·h-1, respectively, P = 0.56). Conclusions: Manipulation of aminoacidemia before resistance exercise via different patterns of intake of protein altered plasma AA profiles and postexercise intracellular signaling. However, there was no difference in the enhancement of the muscle protein synthetic response after exercise. Protein sources producing a slow AA release, when consumed before resistance exercise in sufficient amounts, are as effective as rapidly digested proteins in promoting postexercise MPS.

Keywords
Amino acid delivery; fast and slow proteins; leucine; muscle protein synthesis; Fatigue Case studies; Nutrient interactions;Mineral metabolism;Nutrition Physiology;Metabolism physiology.

Journal
Medicine and Science in Sports and Exercise: Volume 44, Issue 10