Analysis of the rainbow trout solute carrier 11 family reveals iron import ≤ pH 7.4 and a functional isoform lacking transmembrane domains 11 and 12

Citation

Cooper CA, Shayeghi M, Techau ME, Capdevila DM, MacKenzie S, Durrant CJ & Bury NR (2007) Analysis of the rainbow trout solute carrier 11 family reveals iron import ≤ pH 7.4 and a functional isoform lacking transmembrane domains 11 and 12. FEBS Letters, 581 (14), pp. 2599-2604. https://doi.org/10.1016/j.febslet.2007.04.081

Abstract
A Xenopus oocyte heterologous expression system was used to characterise iron transport properties of two members of the solute carrier 11 (slc11) protein family isolated from rainbow trout gills. One cDNA clone differed from the trout Slc11α containing an additional 52bp in the exon between transmembrane domains (TM) 10 and 11. The 52bp contained a stop codon, resulting in a novel isoform lacking the last two TM (termed slc11γ). Slc11γ and another isoform slc11β, import Fe2+ at external pHs ⩽ to 7.4. Trout slc11β Fe2+ import was more sensitive to inhibition by divalent metals. The novel vertebrate slc11γ isoform functions without TM11 and 12.

Keywords
Divalent metal transporter 1 (DMT1); Iron; Natural resistance-associated macrophage protein (Nramp); Fish; Transmembrane domains; Rainbow trout

Journal
FEBS Letters: Volume 581, Issue 14

• MacKenzie_FEBS Letters 2007.pdf