Article
Details
Citation
Matallana-Surget S, Derock J, Baptiste L, Badri H, Deschoenmaeker F & Wattiez R (2014) Proteome-wide analysis and diel proteomic profiling in the cyanobacterium Arthrospira platensis PCC 8005. PLoS ONE, 9 (6), Art. No.: e99076. https://doi.org/10.1371/journal.pone.0099076
Abstract
The filamentous cyanobacteriumArthrospira platensishas a long history of use as a food supply and it has been used by the European Space Agency in the MELiSSA project, an artificial microecosystem which supports life during long-term manned space missions. This study assesses progress in the field of cyanobacterial shotgun proteomics and light/dark diurnal cycles by focusing onArthrospira platensis. Several fractionation workflows including gel-free and gel-based protein/peptide fractionation procedures were used and combined with LC-MS/MS analysis, enabling the overall identification of 1306 proteins, which represents 21% coverage of the theoretical proteome. A total of 30 proteins were found to be significantly differentially regulated under light/dark growth transition. Interestingly, most of the proteins showing differential abundance were related to photosynthesis, the Calvin cycle and translation processes. A novel aspect and major achievement of this work is the successful improvement of the cyanobacterial proteome coverage using a 3D LC-MS/MS approach, based on an immobilized metal affinity chromatography, a suitable tool that enabled us to eliminate the most abundant protein, the allophycocyanin. We also demonstrated that cell growth follows a light/dark cycle inA. platensis. This preliminary proteomic study has highlighted new characteristics of theArthrospira platensisproteome in terms of diurnal regulation.
Journal
PLoS ONE: Volume 9, Issue 6
Status | Published |
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Publication date | 10/06/2014 |
Publication date online | 10/06/2014 |
Date accepted by journal | 09/05/2014 |
URL | http://hdl.handle.net/1893/24033 |
Publisher | Public Library of Science |
eISSN | 1932-6203 |
People (1)
Associate Professor, Biological and Environmental Sciences