Article
Details
Citation
Matallana-Surget S, Baptiste L & Wattiez R (2010) Shotgun proteomics: concept, key points and data mining. Expert Review of Proteomics, 7 (1), pp. 5-7. https://doi.org/10.1586/epr.09.101
Abstract
First paragraph: Mass spectrometry (MS)-based approaches are used increasingly to address diverse questions in proteomics research, enabling one to comprehensively analyze complex samples of proteins on a global level. Owing to all the effort devoted to explore entire genomes, we now have access to a multitude of completed genome sequences, which has recently opened up a new era of ‘shotgun proteome sequencing’. The concept of shotgun proteomics basically consists of the multidimensional separation (MudPIT) of a complex mixture of peptides, generated from the treatment of a specific protease (e.g., trypsin), analyzed using MS/MS and then submitted to automated database searching.
Notes
Output Type: Editorial
Journal
Expert Review of Proteomics: Volume 7, Issue 1
Status | Published |
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Publication date | 31/12/2010 |
Publication date online | 09/01/2014 |
Date accepted by journal | 09/01/2014 |
URL | http://hdl.handle.net/1893/24725 |
Publisher | Taylor and Francis |
ISSN | 1478-9450 |
People (1)
Associate Professor, Biological and Environmental Sciences