Article
Details
Citation
Smith P, Buba E, Desbois AP, Adams A, Verner-Jeffreys D, Joseph A, Light E, Le Devendec L, Jouy E, Larvor E, Boitard P, Jamin M, Keck N, Le Breton A & Thuillier B (2024) Setting epidemiological cut-off values relevant to MIC and disc diffusion data for Aeromonas salmonicida generated by a standard method. Diseases of Aquatic Organisms, 159, pp. 29-35. https://doi.org/10.3354/dao03798
Abstract
The Clinical and Laboratory Standards Institute has published epidemiological cut-off values for susceptibility data generated at 22°°C and read after 44-48 h for florfenicol, oxolinic acid and oxytetracycline against Aeromonas salmonicida. The cut-off values for the minimum inhibitory concentration (MIC) and disc diffusion were derived from data obtained by 1 laboratory and 2 laboratories respectively. The present work reports the generation of susceptibility data from additional laboratories and the calculation of provisional cut-off values from aggregations of these data with previously published data. With respect to MIC data, the provisional cut-off values, derived from aggregations of the data from 4 laboratories, were ≤4 µg ml-1 for florfenicol, ≤0.0625 µg ml-1 for oxolinic acid and ≤1 µg ml-1 for oxytetracycline. For disc diffusion data, the provisional cut-off values derived from aggregations of the data from 5 laboratories were ≥30 mm for florfenicol, ≥32 mm for oxolinic acid and ≥25 mm for oxytetracycline. In addition, a cut-off value of ≥29 mm for ampicillin was derived from the aggregation of data from 4 laboratories.
Keywords
Aeromonas salmonicida; Epidemiological cut-off values; Antimicrobial susceptibility; MIC; Minimum inhibitory; Disc diffusion; Normalised resistance interpretation; ECOFFinder
Journal
Diseases of Aquatic Organisms: Volume 159
Status | Published |
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Funders | Commonwealth Scholarship Commission in the UK |
Publication date | 01/08/2024 |
Publication date online | 01/08/2024 |
Date accepted by journal | 03/06/2024 |
URL | http://hdl.handle.net/1893/36465 |
Publisher | Inter-Research Science Center |
ISSN | 0177-5103 |
eISSN | 1616-1580 |
People (1)
Senior Lecturer, Institute of Aquaculture