Dose-dependency of a combined EPA:DHA mixture on incorporation, washout, and protein synthesis in C2C12 myotubes

Citation

Banic M, van Dijk M, Dijk F, Furber M, Witard O, Donker N, Becker M, Galloway S & Rodriguez-Sanchez N (2024) Dose-dependency of a combined EPA:DHA mixture on incorporation, washout, and protein synthesis in C2C12 myotubes. Prostaglandins, Leukotrienes and Essential Fatty Acids. https://doi.org/10.1016/j.plefa.2024.102651

Abstract
We demonstrate divergent incorporation and washout patterns for EPA and DHA following high and low-dose EPA+DHA incubation in C2C12 myotubes, with higher concentrations favoring n-3 PUFA incorporation. Lower n-3 PUFA concentrations increased MPS without further upregulating the mTORC1 signaling pathway. Our study provides novel insights into the temporal incorporation and washout dynamics of EPA and DHA and, specifically, their combined effect on MPS, thereby advancing knowledge regarding dietary n-3 PUFA prescription to promote skeletal muscle health in humans.

Keywords
Dose; fish oil; mTORC1 signaling; n-3 PUFA; time-course

Notes
Output Status: Forthcoming/Available Online

Journal
Prostaglandins, Leukotrienes and Essential Fatty Acids

• Dose-dependency of a combined EPA:DHA mixture on incorporation, washout, and protein synthesis in C2C12 myotubes