Article

An Efficient Method for the Plating of Haploid and Diploid Emiliania huxleyi on Solid Medium

Details

Citation

Skeffington AW, Grimm A, Schönefeld S, Petersen K & Scheffel A (2020) An Efficient Method for the Plating of Haploid and Diploid Emiliania huxleyi on Solid Medium. Journal of Phycology, 56 (1), pp. 238-242. https://doi.org/10.1111/jpy.12942

Abstract
Emiliania huxleyi is a globally important coccolithophore and one of the most successful eukaryotic organisms in the modern oceans. Despite a large body of work on this organism, including the sequencing of its genome, the tools required for forward and reverse functional genetic studies are still undeveloped. Here we present an optimized method for the clonal isolation of E. huxleyi by plating on solid medium. We demonstrate the utility of this method for a variety of strains including haploid, calcifying-diploid, and noncalcifying diploid strains. We show that, in contrast to previous studies, no changes in cell ploidy status occur when the cells are plated. Our method will greatly aid attempts to elucidate the genetic basis of the remarkable physiology of E. huxleyi by forward and reverse genetic approaches.

Keywords
Coccolithophore; diploid; Emiliania; genetics; haploid; isolation clonal strains; microalgae; plating

Journal
Journal of Phycology: Volume 56, Issue 1

StatusPublished
FundersDeutsche Forschungsgemeinschaft and Boehringer Ingelheim Ltd
Publication date14/02/2020
Publication date online21/11/2019
Date accepted by journal10/10/2019
URLhttp://hdl.handle.net/1893/35335
PublisherWiley
ISSN0022-3646
eISSN1529-8817

People (1)

Dr Alastair Skeffington

Dr Alastair Skeffington

Lecturer in Environmental Genomics, Biological and Environmental Sciences