Article
Details
Citation
Coyle NM, Bartie KL, Bayliss SC, Bekaert M, Adams A, McMillan S, Verner-Jeffreys DW, Desbois AP & Feil EJ (2020) A Hopeful Sea-Monster: A Very Large Homologous Recombination Event Impacting the Core Genome of the Marine Pathogen Vibrio anguillarum. Frontiers in Microbiology, 11, Art. No.: 1430. https://doi.org/10.3389/fmicb.2020.01430
Abstract
Vibrio anguillarum is the causative agent of vibriosis in many species important to aquaculture. We generated whole genome sequence (WGS) data on a diverse collection of 64 V. anguillarum strains, which we supplemented with 41 publicly available genomes to produce a combined dataset of 105 strains. These WGS data resolved six major lineages (L1-L6), and the additional use of multilocus sequence analysis (MLSA) clarified the association of L1 with serotype O1 and Salmonidae hosts (salmon/trout), and L2 with serotypes O2a/O2b/O2c and Gadidae hosts (cod). Our analysis also revealed a large-scale homologous replacement of 526-kb of core genome in an L2 strain from a con-specific donor. Although the strains affected by this recombination event are exclusively associated with Gadidae, we find no clear genetic evidence that it has played a causal role in host specialism. Whilst it is established that Vibrio species freely recombine, to our knowledge this is the first report of a contiguous recombinational replacement of this magnitude in any Vibrio genome. We also note a smaller accessory region of high single nucleotide polymorphism (SNP) density and gene content variation that contains lipopolysaccharide biosynthesis genes which may play a role in determining serotype.
Keywords
Vibrio anguillarum; whole genome sequencing; population structure; recombination; adaptation
Journal
Frontiers in Microbiology: Volume 11
Status | Published |
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Funders | BBSRC Biotechnology and Biological Sciences Research Council |
Publication date | 31/12/2020 |
Publication date online | 29/06/2020 |
Date accepted by journal | 02/06/2020 |
URL | http://hdl.handle.net/1893/31502 |
eISSN | 1664-302X |
People (3)
Research Fellow / Experimental Officer, Sport
Senior Lecturer, Institute of Aquaculture
Post Doctoral Research Fellow, Institute of Aquaculture