Lead Technical Specialist
University of Stirling Pathfoot Building, University of Stirling, Stirling, FK9 4LA
I am the Lead Technical Specialist for the Faculty of Natural Sciences Molecular Biology Team and am based within the Institute of Aquaculture, where I have worked since 1999. My work mainly involves RNA & DNA isolation/quantification, PCR, real-time PCR and next-generation sequencing as well as many sampling trips to the university’s Marine Environmental Research Laboratory at Machrihanish. I have recently developed an in situ hybridisation protocol which allows us to explore the site of expression of various mRNAs in sea lice and salmon tissues and have been involved in establishing protocols for the use of the Institute’s laser capture microscope. I also support the lab practical sessions in the Institute for both undergraduate and postgraduate courses. Previous posts have included working in the Institute’s cell culture/virology facility, research on human hepatitis “B” vaccine escape mutants (Institute of Virology, Glasgow University), on the osmoregulation of marine fish larvae (Department of Biological Sciences, Stirling University) and as an M.L.S.O. in Haematology and Blood Transfusion. I am first author on 2 publications and co-author on 21 others.
Article
Byadgi O, Massimo M, Dirks RP, Pallavicini A, Bron JE, Ireland JH, Volpatti D, Galeotti M & Beraldo P (2021) Innate immune-gene expression during experimental amyloodiniosis in European seabass (Dicentrarchus labrax). Veterinary Immunology and Immunopathology, 234, Art. No.: 110217. https://doi.org/10.1016/j.vetimm.2021.110217
Article
Vogeler S, Carboni S, Li X, Ireland JH, Miller-Ezzy P & Joyce A (2021) Cloning and characterisation of NMDA receptors in the Pacific oyster, Crassostrea gigas (Thunberg, 1793) in relation to metamorphosis and catecholamine synthesis. Developmental Biology, 469, pp. 144-159. https://doi.org/10.1016/j.ydbio.2020.10.008
Article
Vogeler S, Carboni S, Li X, Nevejan N, Monaghan SJ, Ireland JH & Joyce A (2020) Bivalves are NO different: nitric oxide as negative regulator of metamorphosis in the Pacific oyster, Crassostrea gigas. BMC Developmental Biology, 20 (1), Art. No.: 23. https://doi.org/10.1186/s12861-020-00232-2
Article
Fichi G, Carboni S, Bron J, Ireland J, Leaver M & Paladini G (2018) Characterisation of the intracellular protozoan MPX in Scottish mussels, Mytilus edulis Linnaeus, 1758. Journal of Invertebrate Pathology, 153, pp. 99-108. https://doi.org/10.1016/j.jip.2018.02.022
Article
Robledo D, Taggart J, Ireland J, McAndrew B, Starkey W, Haley CS, Hamilton A, Guy DR, Mota-Velasco JC, Gheyas AA, Tinch AE, Verner-Jeffreys DW, Paley RK, Rimmer GSE, Tew IJ, Bishop SC, Bron J & Houston RD (2016) Gene expression comparison of resistant and susceptible Atlantic salmon fry challenged with Infectious Pancreatic Necrosis virus reveals a marked contrast in immune response. BMC Genomics, 17, Art. No.: 279. https://doi.org/10.1186/s12864-016-2600-y
Presentation / Talk
Monaghan S, McNair C, McDonald HC, Ireland J, Hamilton S, Knox D, Roy W, Thompson K, Adams A, Richards R, Smith P, Bassett DI, Matthew C, Preston AC, Groves F, Boyd S, Kanellos T, Asper D & Bron J (2015) Recombinant vaccine efficacy trials against infectious larval salmon lice stages following intraperitoneal immunisation of Atlantic salmon with 9 vaccine candidates. 10th International Sea Lice Conference, Portland, ME, USA, 31.08.2014-05.09.2014. http://sealice2014.businesscatalyst.com/assets/abstract-book-v6.2-final2.pdf
Conference Paper (published)
Carmichael SN, Bekaert M, Taggart J, Ireland J, Christie HRL, Bassett DI, Bron J, Skuce P, Gharbi K & Skern-Mauritzen R (2014) Use of RAD sequencing to isolate a sex-specific SNP marker in the salmonlouse Lepeophtheirus salmonis (Kroyer, 1837). In: Sea Lice 2014: The 10th International Sea Lice Conference Book of Abstracts. 10th International Sea Lice Conference, Portland, ME, USA, 31.08.2014-05.09.2014. Portland, ME, USA: Fish Vet Group, p. 54. http://sealice2014.businesscatalyst.com/assets/abstract-book-v6.2-final2.pdf
Article
Carmichael SN, Bron J, Taggart J, Ireland J, Bekaert M, Burgess S, Skuce P, Nisbet A, Gharbi K & Sturm A (2013) Salmon lice (Lepeophtheirus salmonis) showing varying emamectin benzoate susceptibilities differ in neuronal acetylcholine receptor and GABA-gated chloride channel mRNA expression. BMC Genomics, 14, Art. No.: 408. https://doi.org/10.1186/1471-2164-14-408
Conference Abstract
Herath T, Bron J, Thompson K, Berdal B, Muir K, Ireland J, Auchinachie N, Adams A, Richards R & Taggart J (2013) Transcriptomic analysis of host genes in the heart of Atlantic salmon Salmo salar L in response to salmonid alphavirus (SAV-1). Fish and Shellfish Immunology, 34 (6), pp. 1655-1655. https://doi.org/10.1016/j.fsi.2013.03.065
Article
Herath T, Bron J, Thompson K, Taggart J, Adams A, Ireland J & Richards R (2012) Transcriptomic analysis of the host response to early stage salmonid alphavirus (SAV-1) infection in Atlantic salmon Salmo salar L.. Fish and Shellfish Immunology, 32 (5), pp. 796-807. https://doi.org/10.1016/j.fsi.2012.02.001
Article
Detection of infectious salmon anaemia virus by real-time nucleic acid sequence based amplification
Starkey W, Smail DA, Bleie H, Muir K, Ireland J & Richards R (2006) Detection of infectious salmon anaemia virus by real-time nucleic acid sequence based amplification. Diseases of Aquatic Organisms, 72 (2), pp. 107-113. https://doi.org/10.3354/dao072107
Article
Detection of piscine nodaviruses by real-time nucleic acid sequence based amplification (NASBA)
Starkey W, Millar RM, Jenkins ME, Ireland J, Muir K & Richards R (2004) Detection of piscine nodaviruses by real-time nucleic acid sequence based amplification (NASBA). Diseases of Aquatic Organisms, 59 (2), pp. 93-100. https://doi.org/10.3354/dao059093
Article
Hino K, Basuni AA, Ireland J, Newell A, Mphahlele J, Smit EJ, Ngui SL, Teo CG & Carman WF (2002) Reappearance of hepatitis B surface antigen in immunocompromised individuals: reinfection or reactivation?. Digestive Diseases and Sciences, 47 (2), pp. 415-418. https://doi.org/10.1023/A%3A1013794711235
Article
Nodavirus infection in Atlantic cod and Dover sole in the UK
Starkey W, Ireland J, Muir K, Jenkins ME, Roy W, Richards R & Ferguson H (2001) Nodavirus infection in Atlantic cod and Dover sole in the UK. Veterinary Record, 149 (6), pp. 179-181. https://doi.org/10.1136/vr.149.6.179
Article
Isolation of nodavirus from Scottish farmed halibut, Hippoglossus hippoglossus (L)
Starkey W, Ireland J, Muir K, Shinn A, Richards R & Ferguson H (2000) Isolation of nodavirus from Scottish farmed halibut, Hippoglossus hippoglossus (L). Journal of Fish Diseases, 23 (6), pp. 419-422. https://doi.org/10.1046/j.1365-2761.2000.00254.x
Article
Ireland J, O'Donnell B, Basuni AA, Kean JD, Wallace LA, Lau GK & Carman WF (2000) Reactivity of 13 in vitro expressed hepatitis B surface antigen variants in 7 commercial diagnostic assays. Hepatology, 31 (5), pp. 1176-1182. https://doi.org/10.1053/he.2000.6407
Article
Tytler P & Ireland J (2000) The influence of salinity and temperature change on the functioning of the urinary bladder in the early larval stages of the atlantic herring Clupea harengus L. Journal of Experimental Biology, 203 (2), pp. 415-422. http://jeb.biologists.org/content/203/2/415.short
Article
Failed adoptive immunity transfer: reactivation or reinfection?
Ireland J, Hino K, Lau GK, Cheng C & Carman WF (1999) Failed adoptive immunity transfer: reactivation or reinfection?. Journal of Viral Hepatitis, 6 (1), pp. 73-78. https://doi.org/10.1046/j.1365-2893.1999.6120134.x
Article
Tytler P, Ireland J & Murray L (1997) A study of the assimilation of fluorescent pigments of microalgae Isochrysis galbana by the early larval stages of turbot and herring. Journal of Fish Biology, 50 (5), pp. 999-1009. https://doi.org/10.1111/j.1095-8649.1997.tb01625.x
Article
Tytler P, Ireland J & Fitches E (1996) A study of the structure and function of the pronephros in the larvae of the turbot (Scophthalmus maximus) and the herring (Clupea harengus). Marine and Freshwater Behaviour and Physiology, 28 (1-2), pp. 3-18. https://doi.org/10.1080/10236249609378975
Article
Tytler P & Ireland J (1995) The influence of temperature and salinity on the structure and function of mitochondria in chloride cells in the skin of the larvae of the turbot (Scophthalmus maximus). Journal of Thermal Biology, 20 (1-2), pp. 1-14. https://doi.org/10.1016/0306-4565%2894%2900021-A
Article
Tytler P & Ireland J (1994) Drinking and water absorption by the larvae of herring (Clupea harengus) and turbot (Scophthalmus maximus). Journal of Fish Biology, 44 (1), pp. 103-116. https://doi.org/10.1111/j.1095-8649.1994.tb01588.x
Article
Tytler P & Ireland J (1993) The influence of temperature, salinity and external calcium on diffusional permeability of the eggs of turbot (Scophthalmus maximus). Aquaculture, 115 (3-4), pp. 335-345. https://doi.org/10.1016/0044-8486%2893%2990147-Q
Article
Navarro JC, Ireland J & Tytler P (1993) Effect of temperature on permeability and drinking rates of the metanauplii of the brine shrimp Artemia sp.. Marine Biology, 116 (2), pp. 247-250. https://doi.org/10.1007/BF00350014